Project Summary

The Systems Biology Final Project, investigates the impact of GCNF gene overexpression on human embryonic stem cell differentiation. Leveraging the GEO database and R2GEO for data analysis, the study categorizes embryonic stem cells into undifferentiated and differentiated groups, identifying critical differentially expressed genes (DEGs) with specific thresholds. Techniques like UMAP and Venn diagrams were utilized to reveal DEGs clustering and their biological pathway overlaps, enhancing our understanding of cellular processes involved.

Enrichment analysis further dissected these DEGs, illuminating their roles in cell cycle regulation and differentiation. By constructing protein-protein interaction and gene regulatory networks using Cytoscape, the study highlights central genes within these networks. This project provides insights into stem cell differentiation's genetic mechanisms, focusing on GCNF's regulatory role, and outlines potential therapeutic avenues in developmental biology.