Project Summary

The project explores bulk RNA sequencing from library preparation through to data analysis. The initial stages involve extracting RNA, creating DNA from RNA fragments, adding adaptors, amplifying the library, and performing quality control. Sequencing is carried out using technologies like Illumina, which reads the DNA sequences prepared from the RNA samples.

Data analysis is a crucial part of the study, involving normalization and quality control of data obtained from platforms like Affymetrix and Agilent, highlighted by processes such as RMA normalization and box plotting for Affymetrix, and similar normalization for Agilent with a focus on two-color and single-color options. The analysis progresses to identifying differentially expressed genes (DEGs) through criteria like adjusted p-values and log fold changes, and extends to determine gene biotypes and normalize read counts for further analysis, including network construction and functional enrichment.